Lyophilized peptides require reconstitution before use. The reconstitution process and solvent choice can significantly affect peptide stability and experimental results.
Common Reconstitution Solvents
Bacteriostatic water (0.9% benzyl alcohol in WFI): Most common for in vivo research. The benzyl alcohol inhibits microbial growth, extending stability after reconstitution. Generally compatible with most peptides.
Sterile water for injection (WFI): Standard for in vitro work. No preservative. Use within 7 days of reconstitution.
Acetic acid (0.1-1%): Used for peptides that have poor solubility in neutral water. Common for certain growth factors. Check peptide-specific literature for guidance.
DMSO: Used for highly hydrophobic peptides. DMSO stock solutions are then diluted in aqueous buffer. Check assay compatibility.
Reconstitution Protocol Reference
1. Allow lyophilized peptide to equilibrate to room temperature before opening (prevents condensation)
2. Add solvent slowly along the vial wall, avoid direct stream on powder
3. Gently swirl, do not vortex
4. Allow to dissolve fully before aliquoting
5. Store reconstituted peptide per product-specific guidelines
Calculating Concentrations
For a 5mg vial: adding 1 mL solvent gives a 5 mg/mL (5000 mcg/mL) stock solution. Dilute from stock per experimental protocol requirements.
For educational purposes only. All products are for research use only.